The invention discloses plant formula shampoo capable of effectively relieving itching and removing dandruff and a preparation method of the plant formula shampoo, and belongs to the technical field of production of daily cosmetics. The plant formula shampoo includes a plant composition ingredient, and the plant composition ingredient includes the following components in parts by weight: 10-20 parts of radix sophorae flavescentis, 10-20 parts of fructus cnidii, 8-16 parts of cacumen platycladi, 8-16 parts of herba portulacae, 8-16 parts of folium mori, 8-16 parts of fructus kochiae, 8-16 partsof herba Houttuyniae, 4-12 parts of radix polygoni multiflori, 4-12 parts of radix rehmanniae, 4-12 parts of cortex moutan, 4-12 parts of seed peels of Glycine max (L.) merr., 2-5 parts of dried ginger, 3-10 parts of Lavandula angustifolia, 3-10 parts of radix glycyrrhizae and 2-5 parts of leaves of Chinese Holly. Through compounding of plant components, good compatibility and effects are achieved, the curative effects of dandruff removal and itching relief are improved, the shampoo has safety and reliability, mild performance and no adverse reactions, and the preparation method is simple without complex production devices, has moderate cost, and is suitable for large-scale production.