A realization method of a calibration system of a strapdown inertial measurement unit based on Labview comprises seven steps: 1, arrangement of parameter, 2, controlling of a rotary table, 3, data acquisition, 4, data storage, 5, calibration and solving, 6, verification of calibration results, and 7, display and function of calibration interface. The method improves an automation degree of the calibration of the inertial measurement unit, saves labor and time needed by the calibration experiment, guarantees reliability of the data acquisition and the controlling of the rotary table, and timing accuracy, and has relatively good practical value and broad application prospects in the technical field of inertial navigation.