The invention provides a preparation method and application of an antibiotic
solid-phase extraction material, firstly,
rape pollen is soaked in an
aluminum nitrate solution through an impregnation method, and after
drying, an Al2O3-C template with a developed pore structure is prepared through a
calcination method; then, an Al2O3-C template is used as a matrix for loading LDH, layered LDH is intercalated to the surface of a biological template, and Al2O3-C coated
NiAl-LDH is prepared; and finally, the material is calcined in a
muffle furnace, and the
composite material Al2O3-C-coated
NiAl-LDO with the 3D flower-shaped structure is prepared. The material is stable in property and has good adsorption capacity to the
quinolone antibiotics, the maximum adsorption capacity to the
quinolone antibiotics is 52.28-64.08 mg / g, the
recovery rate can reach 76.4-91.2%, the preparation process is simple, and conditions are mild. The material is used as a
solid-phase extraction material to enrich, separate and detect the
quinolone antibiotics in a
complex matrix, so that the separation selectivity and the detection sensitivity of the quinolone antibiotics are further improved, the efficiency and the reliability of enrichment treatment are improved, the detection process is simple and reliable, the
organic solvent is saved, and the material can be repeatedly used and is environment-friendly.