Bacillus velezensis SF305 with antagonistic effect on hevea brasiliensis and application of bacillus velezensis SF305

A technology of Bacillus Velez and Bacillus, applied in the field of microbial technology application, can solve the problems of difficult gene function research, slow growth rate, etc., and achieve good application potential, broad-spectrum antibacterial and growth-promoting effects

Active Publication Date: 2022-07-12
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The red root pathogen itself contains polysaccharides and polyphenols, and its growth rate is slow, so it is relatively difficult to study the function of its genes

Method used

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  • Bacillus velezensis SF305 with antagonistic effect on hevea brasiliensis and application of bacillus velezensis SF305
  • Bacillus velezensis SF305 with antagonistic effect on hevea brasiliensis and application of bacillus velezensis SF305
  • Bacillus velezensis SF305 with antagonistic effect on hevea brasiliensis and application of bacillus velezensis SF305

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Acquisition of Bacillus velesi SF305

[0042] 1. Soil source

[0043] The orchard soil of Haidian Harbor Garden, Haikou City, Hainan Province, was collected on November 8, 2018.

[0044] 2. Screening of strains

[0045] (1) Soil sample collection

[0046] A soil sample of about 10 cm on the surface of the root circumference of the fruit tree plant was collected, and a soil sample of 200 g was collected at each point. Three soil samples were collected from each plot, and the time, location and type of sampling were recorded. The collected soil samples were stored at room temperature for bacterial isolation.

[0047] (2) Isolation of bacteria

[0048] Plate dilution method: Weigh 10 g of soil sample into a conical flask, add 90 mL of sterile water, shake at 200 rpm and 28 °C in a shaker, take out after 20 min, and let stand for 10 min at room temperature to prepare a soil bacteria suspension stock solution . The stock solution was serially diluted to obta...

Embodiment 2

[0055] Example 2 16S rRNA of Bacillus velesi SF305

[0056] The genomic DNA of strain SF305 was extracted by the Becterial DNA Kit from Omega Company, and the bacterial 16S rDNA gene primers: 27F 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R 5'-TACGGCTACCTTGTTACGACTT-3' were used to carry out PCR with the extracted genomic DNA as a template Amplify to obtain the target fragment. The PCR reaction system is:

[0057] Table 1 Ex-Taq polymerase chain reaction system

[0058]

[0059] The basic conditions of PCR reaction were: 95°C pre-denaturation for 5 min, 94°C denaturation for 1 min, 56°C annealing for 1 min, 72°C extension for 2 min (1 kb / min), 72°C pre-extension for 6 min, and 4°C storage for a total of 30 cycles. After the reaction, the PCR products were checked by 1% agarose gel electrophoresis, and the PCR stock solution was sent to Boshang Biotechnology (Shanghai) Co., Ltd. for sequencing. Sequencing results such as figure 2 shown. DNA Star was used to analyze the sequenc...

Embodiment 3

[0064] Example 3 Physiological and biochemical identification of Bacillus velesi SF305

[0065] The physiological and biochemical characteristics of the Bacillus velesi SF305 of the present invention are: can secrete 3-hydroxybutanone to produce acetomethyl carbinol and gelatinase; cannot hydrolyze o-nitrophenyl-galactoside, arginine, lysine, Ornithine, sodium citrate and sodium thiosulfate, etc.; can not oxidize glucose, mannitol, inositol, sorbitol and rhamnose, etc.; can use glycerol, L-arabinose, ribose, D-xylose, galactose , glucose and fructose and other 20 carbon sources to produce acid, see Table 3 and Table 4.

[0066] Table 3 Physiological and biochemical characteristics of strain SF305 – enzyme activity, carbon source oxidation

[0067]

[0068]

[0069] +: positive reaction; -: negative reaction; W: weak positive reaction

[0070] Table 4 Physiological and biochemical characteristics of strain SF305 - acid production using carbon source

[0071]

[0072...

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Abstract

The invention relates to a bacillus velezensis SF305 strain and an application of the bacillus velezensis SF305 strain. The preservation number of the bacillus velezensis SF305 is CCTCC NO: M 2022129, the bacillus velezensis SF305 has a remarkable inhibition effect on hevea brasiliensis, a good biocontrol resource is provided for prevention and treatment of the hevea brasiliensis red root disease, and the bacillus velezensis SF305 has good application potential in prevention and treatment of the hevea brasiliensis root disease. Meanwhile, the bacillus velezensis SF305 disclosed by the invention has relatively good bacteriostatic activity on phytopathogens such as colletotrichum gloeosporioides of rubber trees, colletotrichum oxysporum of rubber trees, pyricularia oryzae, fusarium oxysporum of cucumbers, phytophthora capsici, botrytis cinerea, xanthomonas oryzae pv. Oryzae, xanthomonas oryzae pv. Oryzicola and the like, and also can promote crop growth, increase the fresh weight of crops and increase the yield of the crops. The strain has the application prospects of disease prevention and growth promotion, and also lays a foundation for research and development of novel microbial agents for diseases such as rubber anthracnose, rice bacterial leaf blight, streak and rice blast.

Description

technical field [0001] The invention belongs to the application field of microorganism technology, in particular to a strain of Bacillus velesi SF305 and its application. Background technique [0002] Natural rubber is widely used in military, aerospace, medical, and automobile manufacturing because of its good elasticity, insulation, strong elongation, good waterproofness, air tightness and wear resistance, and easy bonding with other materials. and other fields, it is an important industrial raw material and strategic material, and its products are as many as 70,000 kinds (Liu Ruijin, Mo Yeyong, Yang Lin, etc. Recognition and Development Suggestions on the Strategic Position of my country's Natural Rubber Industry, China Tropical Agriculture. 2022 (01):13-18). The United States uses natural rubber as a key agricultural raw material, and the European Union includes it in the list of critical raw materials. my country has also actively developed the rubber industry after the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N63/22Y02A40/10
Inventor 涂敏邹丽芳方园伏雪阎依超蔡海滨
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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