The invention relates to a novel fingerprint key generation method for a digital signature, which comprises two stages and is characterized in that the first stage is fingerprint feature extraction which comprises the steps of calculating a directional diagram of a fingerprint image, positioning a reference point of the fingerprint image, calculating a sector fingerprint feature extraction region, carrying out fingerprint image normalization, performing enhancement processing on the fingerprint image, and extracting fingerprint features; and the second stage refers to fuzzy extraction technology based fingerprint key generation which comprises the steps of constructing a security model based fuzzy extractor, constructing a Hamming distance based fuzzy extractor and generating a fingerprint key. The novel fingerprint key generation method has the advantages that the fingerprint key can be cancelled or updated for multiple times, the security is enabled to be higher, and potential safety hazards brought about by external information stealing can be avoided. Meanwhile, the novel fingerprint key generation method has important theoretical and practical values, and has wide application prospects.