The invention belongs to the field of cosmetics, and discloses a stable oil-soluble polypeptide composition, which comprises the following components: polypeptide, a solubilizing agent, and an oily solvent; the solubilizing agent has an octanol water partition coefficient of 1.70 to 1.90 or an HLB value of 8.0 to 10.0; the polypeptide is selected from at least one of palmitoyl tripeptide-7, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, palmitoyl tetrapeptide-5, palmitoyl tetrapeptide-7, palmitoyl tetrapeptide-10, and palmitoyl pentapeptide-4; and the solubilizing agent is at least one selected from the group consisting of ethylhexylglycerin, glycerol caprylate, polyoxyethylene fatty acid, sorbitan laurate, and polyoxyethylene oxypropylene oleate. The oil-soluble polypeptide composition of the present invention is easy to be added to a cosmetic of a full oil formula, and has good compatibility, the stability of the oil-soluble polypeptide composition is good, the product is not easy to grow bacteria, compared with traditional water-soluble peptide products, the product does not need to add preservatives, and the formula is safer.