The invention provides a locomotive intelligent operation optimization calculation method. The locomotive intelligent operation optimization calculation method comprises steps that step 1, information required by locomotive intelligent operation optimization is preprocessed; step 2, an initial optimization curve is generated; step 3, optimization adjustment is carried out based on speed limit; step 4, optimization adjustment is carried out based on time deviation; step 5, the optimization curve is adjusted based on a requirement on operation steady safe shifting, and a final optimization strategy is generated. The strategy of every step is affected by locomotive driving strategy influencing parameters, which are matched with different strategies under different conditions, and are used as strategy classification attribute for strategy classification. The strategy of every step can be used to form a strategy tree, the leaf node of which is the matched final optimization strategy according to the classification attribute. Every step of the whole scheme is capable of satisfying the schedule requirement, the speed limit requirement, the time table requirement, the steady operation requirement, the energy saving requirement, and other requirements of the locomotive operation, and optimization effect and integrated operation efficiency are guaranteed.