The invention discloses a
dextran-based HRP-goat anti-mouse IgG labeled
antibody and a preparation method thereof, and the preparation method is characterized by comprising the following steps: S1, oxidizing
dextran to obtain
aldehyde group-containing
dextran; s2, carrying out
polymerization on the dextran containing the
aldehyde group and
horse radish peroxidase, so as to obtain a
horse radish peroxidase polymer; s3, performing
desalination and
ultrafiltration on the
horse radish peroxidase polymer to obtain an
ultrafiltration product I; s4, connecting a goat anti-mouse IgG
antibody to the
ultrafiltration product I to obtain oxidized
glucan coupled with goat anti-mouse IgG and HRP
enzyme; and S5, purifying the oxidized dextran of the product coupled with the goat anti-mouse IgG and the HRP
enzyme, so as to obtain the dextran coupled HRP-goat anti-mouse IgG labeled
antibody. The
dextran product obtained by the invention is wide in molecular weight coverage, different molecular weights can be selected to design
polymer substances with different sizes, the number of
aldehyde groups can be controlled to control
coupling of different numbers of
protein molecules under the same molecular weight, and the
signal intensity is easy to control.