The invention discloses an isolation door, which mainly includes a door frame, a door leaf, a translational sliding rail, a rotating sliding rail, a sliding seat, a lead screw, a motor, a pair of incomplete gears, a hydraulic cylinder, a cam and a partition. The four door leaves are arranged at 180° Placed side by side, the upper frame of the door is fixed with a hydraulic cylinder support, and the piston rod of the hydraulic cylinder fixed on the hydraulic cylinder support is fixedly connected with the door leaf pull rod. When the piston rod of the hydraulic cylinder stretches and moves, it will drive the door leaf to open and close. The four door leaves After opening, four channels will be formed and have an isolation effect. A lead screw and a sliding seat are installed under the door leaf. After the door leaf is opened, the door leaf can be translated left and right through the sliding seat, thereby adjusting the width of the partitions. A pair of incomplete transmission gears are installed under one side of the door. By controlling the opening of the cam on the pinion shaft to control the lifting of the partition, and then controlling the number and time interval of door leaves moving out, the invention has the advantages of convenient installation, simple operation, and use in various occasions.