The invention provides a catheter feeding device of a puncture needle assembling machine, and belongs to the technical field of medical equipment. According to the device, the problem of poor stability of existing catheter cutting is solved. The catheter feeding device of the puncture needle assembling machine comprises a catheter unwinding mechanism, a catheter taking mechanism and a catheter feeding mechanism, wherein the catheter taking mechanism comprises a base, a positioning assembly, a positioning clamping finger and a cutter assembly, the catheter unwinding mechanism comprises an unwinding support, the catheter feeding mechanism comprises a feeding clamping finger, the catheter taking mechanism further comprises a material taking clamping finger and a cutter buffering block which are arranged on the base in a sliding mode, the feeding clamping finger is located above the end, provided with the material taking clamping finger, of the base and is close to the cutter assembly, theopposite ends of the unwinding support and the base are provided with limiting rolling wheel assemblies, the two limiting rolling wheel assemblies are arranged at the same height and staggered, and the positioning assembly comprises a vertical plate and a catheter inlet guide block which is arranged on the vertical plate and is penetrated through by a catheter. According to the device, cutting stability and cutting precision of the catheter are improved.