The invention discloses an automatic unmanned cashier assembly line, which comprises a first conveying device, a second conveying device, a third conveying device, a fourth conveying device and a fifth conveying device, wherein each conveying device is provided with a fence, and is fixed on the ground by means of a supporting column; each conveying device is provided with a commodity scanning device, a customer places commodities on the conveying device, the commodities automatically advance on an assembly line, the conveying device is provided with a classification baffle, after the commodities are successfully scanned, the commodities are conveyed into a specific conveying belt by means of the baffle, and if the commodities are not successfully scanned, the commodities continue to advance on the conveying belt. The automatic unmanned cashier assembly line can automatically scan the commodities and collecting money of the commodities, is combined with mobile internet payment, saves the labor cost, ensures the cashier efficiency and speed, is attractive in appearance, achieves the whole process of automatic assembly line operation, ensures that each commodity is scanned, considersthe efficiency and speed, is convenient to popularize, and is high in practicability.