The invention relates to a disposable puncture outfit which is a laparoscope supporting surgical instrument suitable for establishing a working channel for an abdominal surgery during laparoscopy and surgery. The disposable puncture outfit comprises a puncture sleeve, a valve body, a gas injection valve, a gas injection switch, a one-way sealing valve, a sealing flat gasket, a self-adjusting sealing cap, a locking fixing cover, a guide sealing cap, a semispherical sealing ring, a locking fixing ring, a positioning guide cover and a puncture cone component, wherein a diagonal plane with taper is arranged on the front part of the puncture sleeve; the outer surface of the puncture sleeve is full of granular bulges; and the puncture cone component comprises a puncture knife, a puncture knife handle, a puncture cone head, a puncture cone lower sleeve, a puncture cone upper sleeve, a puncture cone handle, a trigger safety button and a puncture cone handle cover cap.