The invention relates to a disposable puncture outfit, which belongs to the field of medical instruments, is a surgical instrument matched with a laparoscope, and is suitable for laparoscopic examination and establishment of a surgical working channel. The disposable puncture outfit includes a locking cap, a puncture cannula assembly, a locking sealing cap assembly, a locking switch, an air blocking sealing cap, an air injection valve, an air injection switch, a positioning ring, a one-way valve, a sealing patch and an air bag. The puncture cannula assembly is composed of a puncture cannula seat and a puncture cannula, and the locking sealing cap assembly is provided with a cannula elastic sealing cap, a cannula sealing cap installation seat, a locking fixing cover and a lower pressing cover. A vent groove is formed in the outer side of the puncture cannula, extends forwards to the front portion of the puncture cannula in the axial direction of the puncture tube and passes through the wire binding groove. The air bag is installed at the front end of the puncture cannula and fixed to the periphery of the wire binding groove in a binding mode, and the air outlet hole formed in the sealing patch conveys compressed air for the air bag and inflates the air bag. The positioning ring is sleeved in the middle of the puncture cannula.