Streptomyces variabilis, bacterial agent thereof, and preparing method and application of bacterial agent

A Streptomyces, mutation technology, applied in the field of biological control of microorganisms and plant diseases, can solve the problems of destroying the soil ecological environment, high cost of control technology, unsatisfactory field control effect, etc., to protect the environment, facilitate the expansion of production and application, Significant economic and social benefits

Active Publication Date: 2019-05-14
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of existing plant soil-borne fungal disease control technologies such as high cost or damage to the soil ecological environment, difficult implementation and application, and unsatisfactory field control effects, the present invention provides a strain of Streptomyces mutans that can effectively prevent and control various soil-borne plant fungal diseases , and also relates to its fermentation culture, bacterial agent preparation method and its application in inhibiting soil-borne plant pathogenic bacteria, preventing and controlling soil-borne plant fungal diseases, etc.

Method used

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  • Streptomyces variabilis, bacterial agent thereof, and preparing method and application of bacterial agent
  • Streptomyces variabilis, bacterial agent thereof, and preparing method and application of bacterial agent
  • Streptomyces variabilis, bacterial agent thereof, and preparing method and application of bacterial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Isolation and screening of antagonistic actinomycetes

[0038] The culture medium used in the research is Gao's No. 1 medium: 20g soluble starch, 0.5g NaCl, KNO 3 1g, K 2 HPO 4 ·3H 2 O 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2O 0.01g, agar 20g, water 1000ml, adjust the pH to 7.6, and sterilize at 121°C for 20min.

[0039] Soils from saline-alkali cotton fields and peanut fields in Dongying City, Shandong Province were collected, and actinomycetes were isolated from the saline-alkali soil using Gaoshi No. 1 plate by 10-fold serial dilution separation method, and purified by streaking. Cotton wilt fungus (Fusarium oxysporum f.sp. vasinfectum), cotton Verticillium wilt fungus (Verticillium alboatrum), peanut root rot fungus (Fusarium solani), peanut white sclerotium (Sclerotium rolfsii), solanum blight (Rhizoctonia solani) and other soil-borne plant pathogenic fungi were inoculated on the PDA slope at 28°C for constant temperature cultivation, and the ...

Embodiment 2

[0043] Embodiment 2, identification of antagonistic bacteria

[0044] 1. Culture characteristics and morphological identification of antagonistic bacteria

[0045] Referring to Li Qili's literature (Li Qili. Identification of Streptomyces JK-1 and its disease prevention potential and disease prevention mechanism[D]. Hubei: Huazhong Agricultural University, 2011.29-30), Gaoshi No. 1 agar and inorganic salt starch were prepared respectively. Agar medium (IPS4), yeast extract maltose agar (IPS2), glycerol aspartic acid agar medium (IPS5), oat agar medium (IPS3), potato sucrose agar medium (PSA), refer to the literature of Ruan Jisheng ( Ruan Jisheng, Basics of Actinomycetes Classification, Beijing: Science Press, published in 1977, 142) Preparation of potato cubes and nutrient agar medium.

[0046] The qnsv4 were respectively inoculated into the above-mentioned medium, and cultured in a constant temperature incubator at 28° C. for 7-14 days, and the culture characteristics were ...

Embodiment 3

[0073] Embodiment 3, the solid fermentation of Streptomyces mutans qnsv4

[0074] 1. Configuration of solid fermentation medium

[0075]This embodiment provides a solid medium specially used for cultivating the Streptomyces mutans qnsv4, the solid medium includes a solid substrate, a carbon source, a nitrogen source and inorganic salts; wherein the solid substrate includes corn flour, rice flour, soybean flour, millet flour, and bran; carbon sources include one or more of soluble starch, sucrose, dextrin, maltose, glucose, mannitol, and fructose; nitrogen sources include peptone, yeast extract, One or more of soybean cake powder, urea, sodium nitrate, ammonium sulfate, and ammonium nitrate; inorganic salts include one or more of magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate, and calcium sulfate.

[0076] Wherein, the addition amount (mass) of soluble starch is 4%~6% of solid matrix mass, the addition amount of soybean meal powder is 2-6% of solid matrix ...

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Abstract

The invention discloses streptomyces variabilis, and further relates to a fermentation culture medium, a preparing method of a bacterial agent, and application of the bacterial agent in preventing andtreating soil-borne plant mycosis and the like. The streptomyces variabilis is streptomyces variabilis qnsv4, and the preservation number of the streptomyces variabilis is CGMCC No.16673. The streptomyces variabilis is cultured on a Gauze No.1 culture medium, the bacterial colony is grey white in color and Gram-positive, aerial hyphae grow into spore hyphae after being mature, the spore hyphae are in a spiral shape with 2-8 spirals, generally 4-6 spirals, and spores are spherical or oval. The streptomyces variabilis can grow in an environment with the pH of 6-9 and 0.5-22 g/L of sodium chloride, is tolerant to saline and alkaline, and high in adaptability. The streptomyces variabilis can effectively inhibit the growth of pathogenic bacteria of verticillium wilt of cotton, sclerotium rolfsii of peanuts, fusarium solani of peanuts and other plate soil-borne pathogenic fungi, has a certain effect of promoting the growth of crops and increasing the yield, is beneficial to environmental protection, and has obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of biological control of microorganisms and plant diseases, in particular to a strain of Streptomyces mutans, its bacterial agent, its preparation method and application. Background technique [0002] Plant soil-borne fungal diseases are important plant diseases that are difficult to control. There are many types of pathogenic fungi, such as Fusarium, Sclerotia, Verticillium, Sclerotinia, Rhizoctonia, etc. They live in the soil In the middle, the soil is used as the medium to spread, and when the conditions are suitable, it infects the roots or stems of plants, causing plant diseases such as fusarium wilt, verticillium wilt, white silkworm, and root rot. In particular, various plant diseases such as fusarium wilt, verticillium wilt, root rot, etc., such as cotton fusarium wilt, peanut root rot, and silkworm, often lead to a large number of plant deaths, resulting in severe crop yield reduction or even failure , causi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
Inventor 咸洪泉李雅华崔德杰吕萌
Owner QINGDAO AGRI UNIV
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