The invention discloses a method for improving
autism behaviors through Npas4 expression and overexpression in a cat chat syndrome model, and belongs to the technical field of
animal model construction. Selecting SD wild rats and CdCS model rats, and normally feeding and breeding the rats; the method comprises the following steps: measuring the
protein concentration by adopting a BCA method, then carrying out
Western Blot, then carrying out brain stereotactic injection, and finally carrying out
behavioral testing. According to the invention, a CdCS
rat model is established by using a
CRISPR-Cas9 technology, and the model shows cognitive and
social behavior disorders and medial
prefrontal cortex (mPFC)
neuron dendritic
abnormality; in addition, an abnormally expressed
gene is found in the model mouse, the research focuses on Npas4, and a new insight is possibly provided for
disease treatment.