56 results about "Prochirality" patented technology

The invention discloses a rhodotorula cell and a method for producing optical active alcohols by using the cell. The rhodotorula cell taken as a catalyst can convert substrates of various prochiral ketones under mild reaction condition to obtain corresponding optical active alcohols. The method has high conversion rate of the substrates, high optical purity of products (the enantiomeric excess value is all over 90 percent), and high actual application value and development prospect.

The present invention relates to synthesis of β-amino acid derivatives of formula (I) and its salts of formula (Ia) by a novel process. The process comprises the reduction of a protected or unprotected prochiral β-amino acrylic acid or derivative there of, by using borane containing reducing agents at atmospheric pressure. The resulting racemic β-amino compound is resolved to a pure stereoisomer of formula (I), specifically to (2R)-4-oxo-4-[3-Ctrifluoromethyl)-5,6-dihydrol[1,2,4]triazolo[4,3-alpyrazin-7(8H)-yl]-1-(2,4,4-trifluorophenyl)butan-2-amine. In an embodiment the invention disclosed polymorphic forms of formula (I), phosphate salt of formula (I) and also a Dibenzoyl-L-tartaric acid salt of formula (I).

The invention discloses a chiral beta-hydroxyamide compound and a preparation method thereof, wherein the compound is represented by a formula I. According to the invention, the raw material for preparing a non-natural chiral amino acid compound is obtained by carrying out catalytic hydrolysis on a prochiral diamide compound II with different substituents by using a Rhodococcus erythropolis AJ270microbial system, wherein the use amount of the Rhodococcus erythropolis can be adjusted according to the use amount of a substrate, the reaction solvent is a common buffer solution with the pH valueof 6.0-8.0, the temperature is 20-37 DEG C, the reaction time is 3-120 h, and the Rhodococcus erythropolis microbial catalytic system can be cultured through fermentation and conveniently stored; andwith the application of the biotransformation to prepare chiral monoamide carboxylic acid and dicarboxylic acid, the characteristics of simplicity and convenience in operation, high reaction efficiency, mild reaction conditions, high enantioselectivity, easiness in product separation and high product purity are achieved, and the application prospect is god.

The invention discloses a reductase, a gene of the reductase, a recombinant expression vector and recombinant expression transformant which contain the gene, a recombinase of the reductase, a preparation method of the recombinase, and an application of the reductase or the recombinase thereof used as a catalyst in the asymmetric reduction of a prochiral carbonyl compound so as to prepare chiral alcohols with optical activities. The reductase or the recombinase thereof can derive from Bacillus sp. of which is the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.2549, can be used as the catalyst in the asymmetric reduction of the prochiral carbonyl compound so as to prepare various chiral alcohols with optical activities, and has high catalytic efficiency and strong stereoselectivity; and the applicable reaction conditions are mild and the reductase or recombinase is environmentally friendly. Compared with the intact cells of the wild-type Bacillus sp. of which preservation number is CGMCC No.2549, the reductase has better catalytic effect and higher substrate applicability; and the reductase has very good industrial application and development prospects.

The present invention relates to a process of manufacturing compound having stereogenic centers from a mixture of E / Z isomers of unsaturated compounds having prochiral double bonds. The hydrogenation product has a specific desired configuration at the stereogenic centers. The process involves an asymmetric hydrogenation and an isomerization step. The process is very advantageous in that it forms the desired chiral product from a mixture of stereoisomers of the starting product in an efficient way.

The invention discloses a chiral ferrocene PNNO tetradentate ligand and application of the chiral ferrocene PNNO tetradentate ligand in asymmetric hydrogenation reaction, and belongs to the field of fine chemical engineering. The ligand disclosed by the invention is a novel chiral tetradentate phosphine ligand, and the ligand has the advantages of simplicity in synthesis, easiness in modification, good stability and the like. Metal complexes of the ligands show very excellent catalytic activity and extremely high enantioselectivity in asymmetric hydrogenation reaction of ketone, and alpha, beta, beta, beta-tetracarboxylic acid can be efficiently converted into alpha, beta, beta-tetracarboxylic acid, alpha, beta-tetracarboxylic acid and alpha, beta-tetracarboxylic acid. Prochiral ketones such as alpha, beta-unsaturated ketone, simple aryl alkyl ketone, alpha-hydroxy aryl alkyl ketone, alpha-amino aryl alkyl ketone, alpha-chloro aryl alkyl ketone, beta-keto ester and aryl (hetero) aryl ketone are reduced into corresponding chiral alcohols, and the method has important industrial application value.

The invention discloses a method for synthesizing chiral sulfoxide from thioether catalyzed by Rhodococcus, belonging to the technical field of microbes. Using the resting cells of a strain of Rhodococcus sp.CCZU10-1 with the preservation number CGMCCNo.4911 as a biocatalyst, the chiral S -Benzyl sulfoxide (e.e. >99.9%) and its derivatives. The bacterial strain and the stereoselective biological oxidation method of the present invention not only have good catalytic effect, but also have the characteristics of simple operation process route, mild reaction conditions, no pollution and the like.

The invention discloses an alcohol dehydrogenase mutant and use thereof. The alcohol dehydrogenase mutant of the present invention has high thermal stability and enables high catalytic efficiency and high conversion rate (i.e. space time yield) in the asymmetric reduction of prochiral diaryl ketones to produce chiral diaryl alcohols. Therefore, the alcohol dehydrogenase mutant of the present invention has extremely high prospect of application in the production of chiral diaryl alcohols, such as (S)-(4-chlorophenyl)-(pyridin-2-yl)-methanol, (R)-(4-chlorophenyl)-(pyridin-2-yl)-methanol.

The invention discloses a detection method of a dissociated chiral amino acid in a milk base material. The detection method comprises the following steps: (1) preparing a sample to be detected, namely, removing fat and protein out of the milk base material and extracting a dissociated amino acid to obtain a sample to be detected; (2) deriving, namely, deriving a standard amino acid and the sample to be detected in the step (1) respectively under deriving conditions that N-isobutyryl-L-cysteine and phthalic dicarboxaldehyde are used for combined derivation and an internal standard substance is L-homoarginine; and (3) performing separation detection, namely, performing separation detection of the amino acid by using a high performance liquid chromatography-fluorescence detection method and comparing the chromatogram of the sample to be detected with the chromatogram of a standard amino acid. According to the detection method, the high performance liquid chromatography separation is combined with pre-column chiral reagent derivation and a fluorescence detection method, so that the detection limit is greatly reduced, the resolution ratio and the sensitivity of detection can be improved; and the detection method can be used for qualitative and quantitative analysis of chiral configuration to the amino acid components in the milk base material.

The invention discloses a compound containing chiral imidazole picolinamide. The compound is shown as a formula (I). The invention further discloses a preparation method and application of the compound containing chiral imidazole picolinamide. The compound containing chiral imidazole picolinamide provided by the invention can serve as a catalyst, and is used for preparing a chiral organoboron compound by performing asymmetric hydroboration addition on carbon-carbon atomic double bonds of a prochiral organic compound. The ee value is 99%. The structural formula is as shown in the specification.