The invention discloses a fixing device for an advertisement electronic screen. The fixing device comprises a fixing base and the advertisement electronic screen, wherein the fixing base is fixedly installed in a wall, and the advertisement electronic screen is connected with the fixing base in an inserting fixing fit mode; an inserting fixing groove is formed in the left side inner wall of the fixing base, and spring grooves are oppositely formed in the upper side end wall and the lower side end wall of the inserting fixing groove; jacking springs are fixedly arranged on the positions, deviating from the inserting fixing groove, of the inner end walls of one sides of the spring grooves, the positions, close to the inserting fixing groove, of the end parts of one sides of the jacking springs are fixedly connected to isolation plates, and the positions, deviating from the jacking springs, of one sides of the isolation plates are provided with jacking inclined guide surfaces facing the advertisement electronic screen; an electric connecting groove is formed in the right side inner wall of the inserting fixing groove in a communicating mode; and groove holes are formed in the positions, on the upper side end wall and the lower side end wall of the insertion fixing groove, in the fixing base in a communicating mode. According to the fixing device for the advertisement electronic screen, the advertising electronic screen is prevented from being loosened, normal use of the electronic screen is prevented from being influenced by power failure, accidents caused by falling are prevented, and safety and reliability are achieved.