The invention discloses SO3 concentration testing equipment which comprises a sampling gun, one end of the sampling gun is connected with a heating filter, one end of the heating filter is connected with a sampling pipe, the sampling pipe is sequentially provided with a heating connecting pipe and a first thermometer, and one end of the sampling pipe is connected with a control condensation pipe;the invention further discloses an SO3 concentration testing method which comprises the following steps: S1, performing air suction for 4-6 minutes before sampling, performing sampling by using an airsuction pump, and performing dissolving in an isopropanol solution to obtain the sample solution of which the sampling volume is greater than 0.5 m<3> so as to avoid cleaning interference; and S2, weighing 0.20g of thorium reagent, and dissolving the thorium reagent in 100 mL of deionized water to obtain a thorium reagent solution with the mass concentration of 2000 mg/L. the SO3 concentration testing equipment has the advantages of few interference factors during flue gas extraction, stable extraction, strong processing capability, convenient and fast determination, short period and high determination precision after flue gas extraction, reduction of the labor cost and the time cost of detection, and provision of accurate data for subsequent recording and desulfurization detection work.