The invention discloses bitter almond oil and an extraction method thereof. Bitter almonds are taken as raw materials, a low-temperature and biological processing technique is adopted in the whole process, loss of thermal-sensitive nutritional substances such as protein, higher fatty acid, vitamins and the like in the bitter almonds is effectively avoided, free oil, an emulsion, hydrolysate and almond dregs are obtained after microwave radiation sterilization, frozen-breaking, enzymolysis and vacuum centrifugation, free oil is obtained after the emulsion is subjected to biological demulsification and vacuum centrifugation, all the free oil is combined, and the bitter almond oil is obtained, wherein the bitter almond oil has a high oil extraction rate being 93%-97%, the content of nutritional and active substances is high, the food safety is high, the residual amount of cyanide is under 0.08 mg / kg, and the guarantee period of the bitter almond oil is long and can reach 5-6 years. With the adoption of the extraction method, the quality and the yield of products are improved, meanwhile, the variety, the yield and the additional value of byproducts obtained after the oil is extracted are increased, the extraction cost of the oil is further reduced, discharging of three wastes is avoided, environment protection is realized, and low-carbon production is truly realized.