19 results about "Cystein protease" patented technology

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The present invention pertains to anti-coronaviral compounds. The disclosure includes a method for preventing and / or treating a coronavirus infection through the inhibition of a cysteine protease in a virus, particularly SARS-COV-2. Also provided includes the composition / pharmaceutical composition for preventing and / or treating a coronavirus infection comprising any of the compounds, pharmaceutically acceptable salt thereof, or its mixture, and the use of the compounds.

The invention relates to a method or regimen comprising the administration of IgG cysteine protease to a subject in order to improve the benefit of a subsequent vector-based therapy, where the vector-based therapy treats a disease or condition in said subject. Accordingly, the invention also relates to the treatment of diseases or conditions with a vector-based therapy. The method comprises administering to the subject at least two doses of an IgG cysteine protease; and subsequently administering said vector-based therapy. Methods of the invention are particularly useful for subjects who have pre-existing immunity or pre-existing neutralizing antibodies against the vector-based therapy.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The invention discloses a gene LpOryzain alpha for delaying senescence of perennial ryegrass leaves and application, and belongs to the technical field of plant genetic engineering. According to the invention, a cysteine protease LpOryzain alpha is identified in perennial ryegrass, and the nucleotide sequence of the gene CDS is as shown in SEQ ID NO. 9. The expression quantity of the LpOryzain alpha in the perennial ryegrass is higher along with the aging of leaves. Furthermore, in overexpression of the LpOryzain alpha in arabidopsis thaliana and perennial ryegrass, it is found that the LpOryzain alpha can delay the senescence degree of leaves. Therefore, the perennial ryegrass leaf senescence gene LpOryzain alpha has the function of delaying plant leaf senescence, and an important reference gene and a theoretical basis are provided for improving the quality of turfgrass and pasture.

The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG and in vitro generation of F(ab′)2 fragments.

Provided is a food composition for suppressing muscle fatigue during or immediately after exercise, suppressing sudden muscle pain or increasing muscle strength, and food containing the food composition. The food composition contains a collagen peptide having an average molecular weight of 2,000 or less. The collagen peptide may contain X-Hyp-Gly wherein X is an amino acid residue other than Gly, Hyp and Pro. Such a collagen peptide may be obtained by degrading gelatin with a cysteine protease. The food composition can be used as a food as it is, or may be processed into a food by adding other ingredients thereto. The food composition can suppress muscle fatigue during or immediately after exercise, suppress sudden muscle pain or increase muscle strength.

The invention provides siRNA, a kit and a method for reducing invasion ability of ichthyophthirius multifiliis and application of the siRNA, and belongs to the technical field of molecular biology. The positive-sense strand of the siRNA disclosed by the invention is as shown in SEQ ID NO.5, and the antisense strand of the siRNA is as shown in SEQ ID NO.6. The siRNA can silence expression of cysteine protease genes of the ichthyophthirius multifiliis, and the invasion ability of the ichthyophthirius multifiliis to a host can be remarkably reduced by soaking and transfecting ichthyophthirius multifiliis bodies for 12 hours through the siRNA with the concentration of 30-100 nM; experiments prove that ichthyophthirius multifiliis infected by goldfish body surface tissues transfected with the siRNA is obviously lower than that of a control group. The invention further provides a kit containing the siRNA and a method for applying the siRNA, the siRNA can be used for reducing the invasion ability of ichthyophthirius multifiliis and protecting ichthyophthirius multifiliis hosts, an efficient and reliable molecular tool is provided for ichthyophthirius multifiliis infection prevention and control, and the application prospect is wide.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a corn chromosome 4 white spot disease resistance related molecular marker and application, specifically, the application provides a corn white spot disease resistance related molecular marker, the molecular marker gene is a Zm00001eb168510 gene and / or a sequence as shown in SEQ ID NO:1, a CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO:2, through years of experiments in multiple points, the application is co-located to a white spot disease resistance QTL qMWS4-4 interval and a resistance SNP:4-14146725 and an associated gene Zm00001eb168510 on a corn chromosome 4, the SNP site can be located in different environments through QTL linkage analysis and GWAS correlation analysis, and can explain 15.14% of the phenotype variation; the associated candidate functional gene mainly encodes a cysteine protease superfamily protein, the cysteine protease superfamily contains a deubiquitinating enzyme, and plays an important role in plant development and adversity response.

The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG and in vitro generation of F(ab′)2 fragments.

The present invention relates to a composition and to the production and use of said composition.Pesticide synergist composition containing, among others, (1) a cysteine proteolytic enzyme (or a mixture of cysteine proteolytic enzymes), (2) a polyphenolic compound (or a mixture of polyphenolic compounds chosen from among hydrolysable tannins), (3) a compound chosen from among the triglycerides (or a mixture thereof), (4) a phase change inhibitor chosen from among (ethanol, isopropyl alcohol, benzylic alcohol and the mixtures thereof), (5) an enzyme stabilizer chosen from among the organic peroxides, (6) a non-ionic surfactant, (7) an essential oil chosen from among the essential oils of Melaleuca alternifolia and quinquenervia, (9) a simple reagent such as acetic acid and citric acid to modify / stabilize the pH the present invention may be in the form of a concentrate or an emulsion containing pyrethrins (pyrethrum flower extract), pyrethroids (i) and (ii), and a mixture thereof. said composition is intended for use in managing the resistance associated with the mechanisms of insects for detoxifying insecticides and to improve the efficiency of certain insecticides, in particular certain insecticides such as pyrethrins and pyrethroids (i) and (ii).

The invention provides application of MSC1094308 in preparation of a medicine for preventing and / or treating inflammasome-related diseases, and belongs to the technical field of biological medicine preparation. The Vps4B ATPase inhibitor MSC1094308 can be used for remarkably reducing the activation of cysteine proteinase-1 (Caspase-1) and the interaction between ASC protein and Caspase-1 protein in a mouse bone marrow-derived macrophage (BMDM). Therefore, the invention proves that the MSC1094308 can effectively inhibit the activation of the inflammasome, thereby providing a new means for developing medicines for treating inflammasome-related diseases.

The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG.

A method for detecting a target nucleic acid in a sample, the method comprising the following steps: (a) coupling of an amplification product of the target nucleic acid, wherein said amplification product is generated if said target nucleic acid is in the sample, comprising modified nucleotides having a first reactive moiety to a second reactive moiety that is linked to a cysteine protease activator molecule to produce amplification products of the target nucleic acid labelled with a cysteine protease activator molecule; (b) optionally immobilizing the labelled amplification products on a surface; (c) contacting the optionally immobilized, labelled amplification products with a reversibly inactivated cysteine protease, wherein the catalytic cysteine residue of the reversibly inactivated cysteine protease is blocked by a disulfide bond or a selenylsulfide bond, and reactivating the proteolytic activity of said cysteine protease by said cysteine protease activator molecule by disulfide bond or selenylsulfide bond reduction; (d) contacting the reactivated cysteine protease with a cysteine protease substrate, wherein said substrate provides a detectable signal when hydrolysed by said reactivated cysteine protease; and (e) monitoring said detectable signal, wherein the presence of the detectable signal is indicative of the presence of said target nucleotide sequence in said sample.

Provided is an IdeS protein variant having IgG-cleaving cysteine protease activity and having at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to SEQ ID No. 2, and (a) having a glutamic acid (E) at a position corresponding to position 226 of SEQ ID No. 1, or (b) having an arginine (R) at a position corresponding to position 167 of SEQ ID No. 1 and a glutamic acid (E) at a position corresponding to position 226 of SEQ ID No. 1. Also provided are a nucleic acid encoding the IdeS protein variant, a vector comprising the nucleic acid, and use of the IdeS protein variant for treating or preventing IgG-related diseases or disorders, or use thereof as a tool enzyme of biotechnology in vitro.

The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG.

The present invention relates to methods for enhancing an adoptive cell transfer immunotherapy that targets an immunoglobulin light chain by administering a protein that has IgG cysteine protease or IgG endoglycosidase activity.

Provided are improved cysteine proteases for specifically cleaving and inactivating immunoglobulin G. The improved cysteine pro-teases are useful in methods of treating diseases, disorders or conditions characterized by excessive levels of IgG antibodies, including autoimmune disorders and candidates for organ transplantation sensitized with anti-HLA antibodies, as well as for treating candidates for gene therapy with pre-existing neutralizing antibodies against recombinant vectors and re-dosing of subjects previously treated with a gene therapy vector.

The invention discloses a synthetic organic nutrient solution based on animal keratin cleavage small peptide, and particularly relates to the technical field of agricultural biologication.The synthetic organic nutrient solution is specifically prepared from a 200-1500 Da small peptide component obtained by directional cleavage from animal keratin through a composite enzymolysis stepped temperature control technology; the plant essential nutrient elements are synergistically stable with the organic acid chelating agent; the triple biological activation system is composed of alginate oligosaccharide, auxin and defense signal molecules; and a space stable structure is formed by xanthan gum-sodium alginate composite colloid. Keratinase and cysteine protease are used for cooperatively breaking a disulfide bond network, and active peptide fragments are enriched through ultrafiltration and grading; component precipitation failure is prevented through organic acid chelation and a colloid three-dimensional network; and integrating biological signal molecules to synchronously activate crop growth and stress resistance pathways. According to the invention, high-valued conversion of waste keratin resources is realized, and the total-effect organic nutrient solution with nutrition delivery, physiological regulation and system resistance induction functions is formed.