The invention discloses a super-resolution imaging method and
system based on a
laser confocal microscope, and particularly relates to the technical field of
laser confocal microscopes, and the method comprises the steps: writing a point detection
signal matrix and a frame index into a
video memory at a collection end through a shared cache in a zero-copy manner; reasoning the real-
time data stream by using a residual network of an integrated
point spread function
convolution kernel to generate a reconstructed
voxel stack; calculating the
signal-to-
noise ratio, the resolution and the drift, converging the
signal-to-
noise ratio, the resolution and the drift into a quality index vector, and comparing the quality index vector with a threshold value to obtain a dynamic scanning parameter vector; the scanning step
pitch and the
laser power are adjusted in real time according to the synchronous mark, and an acquisition-reconstruction
closed loop is formed; performing inter-
frame difference on the reconstructed
voxel stack to obtain a difference
voxel stream, generating an incremental code through adaptive entropy coding, packaging the incremental code and control
metadata into a compressed packet, and directly mapping the compressed packet to a rendering buffer to realize synchronization of
data compression, parameter recording and three-dimensional
visualization; and the calculation time
delay and the storage pressure are remarkably reduced while the high-resolution
image quality is kept.