The invention provides a locking device for a deep-sea manned submersible hatch cover. The locking device comprises a rotary shaft penetrating through the hatch cover. A sealing mechanism is arranged among the hatch cover, a shaft sleeve on the hatch cover and the rotary shaft. The two ends of the rotary shaft are provided with an external rotation hand wheel and an internal rotation hand wheel respectively. The rotary shaft is provided with a transmission threaded section, a traction nut is installed on the transmission threaded section, and two to four connecting rod mechanisms are distributed on the traction nut in the equal included angle mode. Each connecting rod mechanism comprises a short traction rod, a long traction rod, a locking rod and a supporting rod, wherein one end of each short traction rod is hinged to the traction nut, one end of each long traction rod is hinged to the shaft sleeve on the hatch cover, the other end of each short traction rod and the other end of the corresponding long traction rod are hinged to the rear end of the corresponding locking rod, the front middle portion of each locking rod is hinged to one end of the corresponding supporting rod, the other end of each supporting rod is hinged to the hatch cover, and the front end of each locking rod is a locking end. According to the locking device, a pressure-resistant shell structure is not damaged, even and reliable pressing force can be provided, and the locking device is used for locking the deep-sea manned submersible hatch cover.