The invention discloses an on-line 
test structure for 
polycrystalline silicon Poisson ratio. The on-line 
test structure comprises an insulating substrate, an asymmetric polycrystalline cross beam, a first polycrystalline 
lower pole plate and a second polycrystalline 
lower pole plate, wherein the asymmetric polycrystalline cross beam is deflected in an electrostatic 
driving mode, so Poisson ratio parameters of a 
polycrystalline material are obtained according to a geometrical relationship and a material 
mechanics principle. The 
test structure rotates under the action of electrostatic force which is generated through excitation 
voltage, a maximum torsion angle becomes known quantity through structural design, and the Poisson ratio of the 
polycrystalline material is computed according to a measurement value of the excitation 
voltage when the test structure reaches the maximum torsion angle and unknown structure geometric parameters and physical parameters. Therefore, requirements for 
test equipment are low, a test method is simple, and the test process and test parameter values are stable. During synchronization of the 
polycrystalline silicon preparation process and the manufacturing process of a subsequent micro electro 
mechanical system (MEMS), special 
processing requirements are eliminated, and requirements for on-line test are completely met.