The invention relates to a speed-adjustable stirring tank with cooling and temperature sensing functions, which comprises a tank body, a cooling layer is arranged on the outer side of the tank body, adischarging pipe, a steam inlet and a valve switch are arranged below the tank body, an air filter and an air inlet are arranged on the right side of the discharging pipe, and a sampling pipe is arranged on the left side of the tank body, a temperature sensor control device and a cooling water inlet are arranged below the sampling pipe, a biosensor is arranged on the right side of the tank body,a cooling water outlet is arranged above the biosensor, a filter screen is arranged in the tank body, a stirring shaft and stirring blades are arranged above the filter screen, and a speed regulatingdevice and a stirring motor are arranged above the stirring shaft; the upper end of the tank body is provided with a nutrient adding port, an observation hole, an exhaust pipe, an exhaust pipe, a PH meter and a dissolved oxygen detector. By arranging the temperature sensor control device, the cooling layer and the speed adjusting device, the problems that a common stirring tank does not have a cooling function, real-time temperature sensing cannot be conducted, and the stirring speed cannot be conveniently adjusted are solved.