The invention discloses an NK (Natural Killer Cell) immune cell in-vitro expansion kit. The NK immune cell in-vitro expansion kit comprises a box cover, a handle, an outer box body, threaded rods, connecting bearings, an inner box body, lifting sliding blocks, sliding grooves, a glass panel, a bearing plate, test tube inserting holes, through holes, bottom supports, rotating motors, a base, a heating plate, bottom grooves and a damping bottom pad. The NK immune cell in-vitro expansion kit disclosed by the invention has the beneficial effects that the inner box body moves up and down in the outer box body under the interaction of the threaded rods and the lifting sliding blocks which are arranged at two sides, the position of the inner box body can be freely adjusted, the glass panel is insliding connection with the inner box body through the sliding grooves and is used for controlling the opening size of the top part of the inner box body while taking test tubes placed in the inner box body, other test tubes placed in the inner box body can be prevented from being polluted due to too large opening, the test tube inserting holes and the through holes are in one-to-one correspondence arrangement, and gaskets are arranged on the inner walls of the test tube inserting holes, so that the test tubes can be conveniently placed, meanwhile, the placement stability of the test tubes canbe increased, and NK immune cells in the test tubes can be prevented from spilling.