The invention provides an isolation mechanism. The isolation mechanism comprises a rotary shaft, grain, a rotary wheel, an installing seat, an action source and a rotary motor, wherein a cylindrical cavity is formed in the installing seat; the rotary shaft is vertically and coaxially fixed in the cavity and fixedly provided with the rotary wheel; the rotary motor is fixed at the bottom end of therotary shaft and drives the rotary shaft to rotate; the grain is fixed to the position, near the edge, inside the rotary wheel in a penetrating mode; and the action source is arranged at the position,corresponding to the lower portion of the grain, in the installing seat. The rotary motor provides driving force, reverse rotation driving of the rotary wheel is realized, the isolation mechanism ofa device can conveniently restore to the initial state after two-level safety is relieved, the driving force on the rotary wheel at the initial stage and the ending stage is uniform, the rotary wheelis effectively driven to rotate in place, and the reliability of relief of two-level safety is improved. An electromagnetic clamp device is used for conducting two-level safety restraining on the isolation mechanism, and the relief performance of two-level safety of the isolation mechanism can be detected without carrying out a destructive test.