The invention provides a spallation neutron target with a mixed cooling flow channel. The spallation neutron target has a serial cooling flow channel and a parallel cooling flow channel communicated with each other at the same time is provided, aims at thermal deposition of a target body, and takes an internal cooling flow channel as an optimization object. The spallation neutron target comprises a target container and a target body installed in the target container, and a target window is arranged on the front end face, right facing the proton beam bombardment direction, of the target container; the target body is formed by fixedly connecting a plurality of target piece single bodies in a stacked mode, a single serial cooling flow channel is formed by gaps between the target pieces close to the target window direction and the upper inner wall and the lower inner wall of the target container, a parallel cooling flow channel is formed by gaps between connecting faces between every two adjacent connected target pieces far away from the target window direction, and a serial cooling flow channel and the parallel cooling flow channel are communicated with each other. The overall flow of a working medium in the overall target structure is low, high-flow-rate heat dissipation is achieved in a high-heat-flow area of the target body through serial flow, flow resistance is reduced in a low-heat-flow area through parallel flow, efficient cooling and stable flowing are achieved, and therefore the service life of the target body is prolonged.