The invention is applicable to the technical field of fragrance products, and provides a fragrance spray for helping sleep and a preparation method and application thereof. The fragrance spray comprises the following components: herba selaginellae, caulis polygoni multiflori, herba lophatheri, herba mimosae pudica, radix valerianae and lotus plumule. The fragrance spray provided by the invention takes herbal traditional Chinese medicine extract essence as a main component, achieves the purpose of improving sleep by improving the sleep environment, has no toxicity, no harm and no side effects on a human body, overcomes the defects of drug accumulation, drug dependence, drug resistance and other side effects on the human body caused by oral administration of traditional hypnotic drugs, and also overcomes the inconvenience of decoction and administration of traditional Chinese medicines. At the same time, compared with products with sleep improvement, such as aromatherapy products, sachetsachets, medicine bags and medicine pillows on the market, the fragrance spray has the characteristics of no need of heating, no mildew and no breeding of microorganisms, and realizes the product characteristics of portability, convenient use, no side effects, safety, reliability and outstanding effect at home, in travel, in the field and the like.