The invention discloses an audio output regulation and control method and device, and a computer readable storage medium. The method comprises the following steps: monitoring an output instruction of audio data; if the output instruction is monitored, detecting ambient sound in preset time; according to audio features of the ambient sound, determining a corresponding regulation and control coefficient; and responding to the output instruction according to the regulation and control coefficient, and adjusting the amplitude of a volume key according to the regulation and control coefficient. According to the audio output regulation and control scheme provided by the invention, on one hand, volume control of mobile equipment is more user-friendly, i.e., identification adjustment can be performed according to gear or environment, the operation is convenient and fast, and on the other hand, special adjustment by users is supported, the device stores regulation and control preferences of sound source users, regulation and control experience is better, when a mobile terminal performs multi-audio output, different audio output can be respectively prepared and conveniently controlled according to the volume and scene of current output audio.