The invention discloses a method for constructing an atherosclerotic vulnerable plaque mouse model. The method is to obtain a low-density lipoprotein receptor gene (LDLR) knockout mouse with a heterozygous point mutation of the fibrillin 1 (Fibrillin-1, Fbn1) gene by crossing. The present invention for the first time Fbn1 C1039G+ / ‑ Point mutations introduced into LDLR ‑ / ‑ Mouse genome, combining the characteristics of the two genotypes, the method is simple and easy, and can form a large lipid core, a large number of inflammatory cells, a typical thin fibrous cap, abundant new blood vessels and intraplaque hemorrhage, outward remodeling of blood vessels, etc. The characteristic vulnerable plaque, and it can be observed in the model that it is similar to human plaque rupture, which leads to the occurrence of acute cardiovascular and cerebrovascular events. It has a high molding rate and good repeatability, providing a good animal basis for drug research and improvement of device consumables, and a good platform for exploring the mechanism of atherosclerosis.