The invention discloses a vine seedling cultivation box for vine planting, and relates to the technical field of vine planting. The vine seedling cultivation box includes a box body, three support plates are arranged, the left and right side faces of the inner wall of the box body are fixedly connected with the left and right side faces of the support plates, placement grooves are formed in the upper surfaces of the support plates, bearings are in clamped connection with the left sides of the upper surfaces of the support plates, rotation shafts are sleeved with the bearings and are fixedly connected with the bottoms of threaded columns, first handles are fixedly connected to the tops of the threaded columns, threaded nuts are in threaded connection with the outer surfaces of the threadedcolumns, and tilting prevention frames are fixedly connected to the right side faces of the threaded nuts. According to the vine seedling cultivation box for vine planting, by arranging the threaded columns, the rotation shafts, the bearings, the threaded nuts, sliding sleeves and the tilting prevention frames, the heights of the tilting prevention frames can be adjusted as required, so that the situation is avoided that seedlings are not matched with the tilting prevention frames due to the growth of the seedlings, the tilting prevention effect is improved, and the usage requirement is met.