The invention discloses a keypad based on genomic sequence proofreading. The keypad adopts the technical scheme that the keypad comprises a keyboard and an interface, wherein the keyboard comprises 15 keys which are A, T, G, C, S, V, up arrow, down arrow, left arrow, right arrow, Tab, Control, del, enter and space key. The interface adopts a USB (universal serial bus) interface. The 15 keys forms 3 lines and 5 rows, wherein the first line comprises Tab, S, T, up arrow and del from the left side to the right side in sequence; the second line comprises control, A, down arrow, G and left arrow from left side to the right side in sequence; the third line comprises the space key, V, C, right arrow and Enter from the left side to the right side in sequence. The keypad ensures that the genomic sequence proofreading operation is more convenient, and the input efficiency is improved.