The invention discloses a multi-
photon mammary gland spectral imaging and transmission and reflection fusion imaging method, which specifically comprises the following steps: sequentially transmitting three incident lights with different wavelengths and the same incident intensity to the
mammary gland on two sides according to a
time sequence, and collecting
transmission intensity two-dimensional published data after each single-
wavelength incident light penetrates through the
mammary gland on two sides; calculating an
oxyhemoglobin saturation variable quantity
delta Oxy and
a hemoglobin concentration variable quantity
delta ODBV between the bilateral breast tissues corresponding to each pixel, and generating a two-dimensional functional diagram of the
oxyhemoglobin saturation variable quantity and a two-dimensional functional diagram of the
hemoglobin concentration variable quantity by using a computer; incident light with three wavelengths is used for simultaneously transmitting the mammary gland on the affected side, and a transmission original image obtained after the incident light penetrates through the mammary gland is collected; the method comprises the following steps of: irradiating the surface of the transmitting side of the mammary gland on the affected side by using first-area near-
infrared light and second-area near-
infrared light according to a
time sequence, and collecting a shallow
surface layer vascular graph and a deep
surface layer vascular graph which are obtained after the two near-
infrared light are reflected by the mammary gland; and fusing the two
blood vessel images with the transmission original image to obtain a
mammary gland tissue form
fusion image.