The invention discloses a multi-
system coding and reading method for compound base
DNA (deoxyribonucleic acid) storage, which combines multi-
system error correction coding and
symbol mapping methods to code digital information and map the digital information into
a DNA chain containing a compound base and a standard base, and adopts a high-reliability short grouping error
correction code to code an address sequence and add the coded address sequence to two ends of a data
DNA sequence; when the data are read out, a short grouping error
correction code is utilized, a unique address of a sequencing read section is achieved, rapid clustering is achieved, entropy-driven set division and maximum likelihood-based letter detection are combined, original characters of each synthesis site are accurately detected, and
original data are recovered through error correction decoding. According to the method, the separable characteristic of the information entropy is utilized, the composite bases are subjected to diversity, the distinction degree between the concentrated characters is increased, the detection capacity is enhanced, the
base frequency information in the
sequencing data can be effectively utilized, and the coverage degree needed by composite base detection is reduced.