The invention relates to an antibacterial and detackifying agent, a preparation method thereof and a method for improving the hatching rate of artificial breeding of crocodile vacheronii by using the antibacterial and detackifying agent, belonging to the technical field of aquaculture. In order to solve the problem of low hatching rate and low survival rate of crocodile vacheronii artificial breeding, the invention provides a kind of antibacterial debonding agent, which mainly consists of an adsorptive carrier and an antibacterial component loaded on the carrier. The antibacterial The ingredients are mainly composed of gallnut, skullcap, flavescens, honeysuckle, forsythia and calamus. The method for improving the hatching rate of artificial breeding of crocodile vacheronii by using the antibacterial and debonding agent of the invention includes artificial fertilization, debonding of fertilized eggs, treatment of saprolegnia prevention, hatching and seedling raising. The antibacterial and debonding micron-level adsorptive carrier of the present invention has a good debonding effect, which enables the fertilized eggs to be separated from each other and improves the hatching rate of the fertilized eggs; the active ingredients of the traditional Chinese medicine loaded on the adsorptive carrier can inhibit Saprolegnia spores and other bacteria The invasion of saprolegniasis is prevented, and the survival rate of fry is improved.