The invention relates to a formula of an anti-stress extruded floating feed for megalobrama amblycephala and a preparation method thereof, belonging to the field of fish feed and preparation thereof. The anti-stress extruded floating feed for the megalobrama amblycephala, which comprises the following components by weight percent: 10-16% of fish meal, 15-30% of extruded soybean, 10-20% of rapeseed meal, 4-10% of peanut meal, 6-12% of cottonseed meal, 8-12% of corn protein powder, 10-18% of wheat shorts, 1-5% of squid paste, 0.25-0.75% of 50% choline chloride, 0.1-0.5% of acanthopanax root, 0.01-0.1% of pilose Asiabell root, 0.01-0.05% of Chinese magnoliavine fruit, 0.02-0.08% of schefflera arboricola hayata, 0.1-1% of spine date seed, 0.01-0.08% of jatamans valeriana rhizome, 0.5-1.5% of vitamin premix, 0.5-1.5% of mineral premix, 2-5% of calcium hydrophosphate, 1-2% of fish oil and 0.5-1.5% of lecithin oil. The anti-stress extruded floating feed for the megalobrama amblycephala can improve the immunity and the oxidation resistance of the megalobrama amblycephala, improve the stress and the anti-disease capability, improve the palatability of the feed, promote the growth and improve the survival rate of the megalobrama amblycephala.