The invention relates to a method for preparing nicotinamide-coated multivesicular liposomes. The nicotinamide-coated multivesicular liposomes are prepared from phospholipid, neutral lipid, cholesterol, an organic solvent, a hydrophilic emulsifier, nicotinamide, amino acid, an osmotic pressure regulator and deionized water. The method comprises the steps: dissolving the phospholipid, the cholesterol and the neutral lipid in the organic solvent, so as to obtain an organic phase; uniformly mixing the nicotinamide, the amino acid, the osmotic pressure regulator and the deionized water, so as to obtain an internal water phase; homogenizing the organic phase component, and adding the internal water phase component into the organic phase component, so as to prepare a W/O primary emulsion; mixing the hydrophilic emulsifier, the amino acid, the osmotic pressure regulator and the deionized water, and carrying out dispersing, so as to obtain an external water phase; homogenizing the external water phase component, adding the W/O primary emulsion component into the external water phase component, carrying out homogenizing, then, carrying out ultrasonic treatment, and then, cooling the mixture to room temperature with stirring, so as to form a W/O/W multiple emulsion; dispersing the multiple emulsion into an amino acid solution, introducing nitrogen gas into the solution to remove the organic solvent, thereby obtaining a nicotinamide-coated multivesicular liposome suspension. According to the method, the stability of the nicotinamide is improved.