The invention discloses a tracked vehicle gun aiming method based on line-of-sight tracking. The tracked vehicle gun aiming method based on line-of-sight tracking aims to overcome the problems of lowapplicability, low precision and unsatisfactory real-time performance in the prior art, and comprises the following steps of: 1) acquiring a human eye image, wherein after a line-of-sight tracker is correctly worn, the position of a human eye CCD camera relative to the center of two eyes is adjusted, and the human eye CCD camera faces right towards the front direction so as to ensure that the line-of-sight tracker can accurately complete acquisition of the eye image of a shooter; 2) pupil detection, wherein (1) eye image preprocessing, (2) pupil center positioning, (3) pupil tracking, (4) Pulchin facula center positioning and (5) P-CR vector obtaining are carried out; 3) fixed-point calibration; 4) line-of-sight tracking, wherein after the mapping relationship between a P-CR vector and thecoordinates of a point of sight of a scene image is obtained, eye movement parameters of a new human eye image are further obtained, and then the position of the point of sight in the scene image isestimated by means of a determined mapping relationship to complete final line-of-sight tracking; and 5) executing control instructions.