The invention discloses a printing medium positioning and printing method, device and equipment, a medium and a flat-bed printer. The method comprises the following steps that reference image data of the positioning printing medium are obtained, a reference image is printed on a printing platform according to the reference image data, each printing medium is positioned according to the reference image, position information of each printing medium in the reference image is obtained, a to-be-printed image corresponding to each printing medium is subjected to type setting according to the position information, and ink-jet printing is conducted on the printing media according to the to-be-printed images subjected to type setting. The method is accurate in positioning, high in efficiency and low in cost, no extra positioning template is needed for printing and positioning, the reference image can be used for positioning various printing media, flexibility and convenience are achieved, the reference image can be used permanently through one time printing, the problem that the positioning templates are customized for different printing media each time is solved, and especially for batch printing, the method can accurately and quickly realize positioning and printing.