The invention discloses a high-resolution water-based flexible printing ink comprising ink paste and acrylic emulsion. The ink is characterized in that the ink paste comprises the following components in parts by weight: 20 to 30 parts of water-based acrylic resin, 30 to 60 parts of pigment, 2 to 8 parts of dispersant 760W, 10 to 20 parts of water, 10 to 15 parts of ethanol, and 0.5 to 1 part of defoaming agent. The invention further discloses a preparation method for the ink. The quantity of the pigment is increased in the ink paste of the ink disclosed by the invention, and meanwhile, the dispersant 760W is adopted, so that a large dose of pigment can be well dispersed, and the ink has good stability and good dyeing property. The ink can reach more than 100 resolution cables and can be used for directly printing line boards, and the inking effect is similar to an offset printing effect. The third dimension of printed pictures can be reflected, and the pictures are exquisite and vivid. Moreover, the ink disclosed by the invention is water-based ink, so the ink accords with the national environmental protection policy, and can achieve the printing effect of an oil-based ink.