The invention provides an anoxia-responsive nano
drug carrier and a preparation method and application thereof. The nano
drug comprises a hydrophobic seed core and an amphiphilic shell, wherein the hydrophobic seed core comprises
chlorin e 6 and tirazamine, and the amphiphilic shell is hydrophilic macromolecular
hyaluronic acid modified by hydrophobic molecule 2-
nitroimidazole, the hydrophobic seed core and the amphiphilic shell form a non-conjugated position relationship by hydrophilic and hydrophobic forces. According to the preparation method, the nano
drug is finally successfully preparedby optimizing the preparation process, the particle size of the product is moderate and controllable, the nano drug is irradiated by
laser for photodynamic treatment,
oxygen is consumed, and hydrophobic molecules 2-
nitroimidazole is transformed into hydrophilic molecules 2-aminoimidazole under the anoxic condition, and the nano drug is dissolved, the
drug release is achieved, the
chemotherapy toxicity of the chemotherapeutic drug tirazamine is activated under the anoxic condition, the
combined treatment is synergistic,
cancer cells are effectively killed, the application prospect is wide and the market value is huge.