According to the invention, the multi-mode time-
frequency domain conversion and the
deep learning technology are combined, and a multi-component mixed Raman spectrum unmixing method is developed, so that clinical in-vivo and in-situ detection and
disease diagnosis of novel Raman probes, instruments and the like are facilitated. The method comprises the following steps: (1) converting a mixed Raman spectrum from a
time domain to a
frequency domain by using
fast Fourier transform (FFT),
discrete cosine transform (DCT) and
discrete sine transform (DST), and extracting
frequency domain features; (2) extracting multi-scale local time-frequency domain characteristics of the
mixed spectrum by using short-time
Fourier transform (STFT) and
discrete wavelet transform (DWT); (3) carrying out spectral unmixing calculation in each mode in combination with a one-dimensional attention mechanism U-shaped neural
network model; and (4) fusing various
modal unmixing results by using a meta-learning method, and analyzing the weight of each
modal to obtain an accurate unmixing spectrum. Compared with a traditional Raman
spectrum analysis method, the Raman spectrum multi-component
signal unmixing method based on multi-
modal time-frequency
domain transformation and
deep learning can accurately separate independent Raman signals of different tissue structures and biochemical components in a complex environment in a
living body, so that the unmixing accuracy of the Raman spectrum multi-component
signal is improved. Therefore, convenience is provided for subsequent
disease mechanism analysis and diagnosis. The method provides an innovative and potential solution for in-vivo and in-situ detection analysis and
disease diagnosis of medical clinical
Raman spectroscopy.