The invention discloses a membrane isolating type oil storage and transportation device, comprising a casing, an oil inlet pipe, an oil outlet pipe and a breathing hole, wherein the casing is internally provided with a membrane the volume of which is larger than that of the casing, the top part of the membrane is partially fixed on the top part of the casing, the rest part of the membrane is separated from the casing, and the inner layer of the membrane is communicated with the breathing hole and isolates the inner cavity of the casing from air; and the oil inlet pipe and the oil outlet pipe are arranged on the top part or the bottom part of the casing, are communicated with the inner cavity of the casing and are all provided with a valve and a quick connector respectively, and the top part of the casing is provided with a safety valve. The membrane isolates storage objects from air, thus eliminating breather loss, protecting environment, reducing oil oxidization, avoiding water from being mixed with oil, removing oil steam mixed with air so as to improve safety of storage and transportation, and reducing risks of fire and explosion occurrence. Due to the flexible structure, the invention is favor of resisting external force influence such as impact and the like, is suitable for transformation of vertical and horizontal oil tanks, vertical oil tank and standby reservoir, wherein the transformation is simple and feasible, has the advantages of environment protection, safety and energy conservation, and can eliminate the majority of current oil steam pollution after being implemented.