43results about How to "No false negative results" patented technology

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.

The invention discloses a CYP2C19 gene polymorphyism detection kit and a detection method thereof. The kit comprises ultrapure water, a solution X, a 10*PCR (polymerase chain reaction) buffer solution, PCR primers, a 25mM magnesium chloride solution, DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the PCR primers include a different genotypic forward and reverse amplification primer, an internal DNA reference forward and reverse amplification primer and an internal reaction reference forward and reverse amplification primer on three SNP (single nucleotide polymorphism) sites of a CYP2C19 gene; and gene sequences of the primers are shown as SEQ ID NO. 1-NO. 13 (sequence identifier number 1-number 13). The detection method comprises the steps of collecting a sample, extracting nucleic acid, taking nucleic acid of a patient as a template to conduct PCR reaction, and using a GeXP genetic analyzer to conduct capillary electrophoretic separation on the sample. The detection kit and the detection method have the advantages of high specificity, high sensitivity, high flux, high reliability, low cost and no false negative results.

The invention discloses a kit for synchronously detecting twenty-three meningitis pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the twelve meningitis pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-13 (sequence identifier number 1-13), and the PCR primer comprises forward and reverse PCR amplification primers of the rest eleven meningitis pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the twelve meningitis pathogens and the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 14-52. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention discloses a kit for synchronously detecting twenty-two respiratory tract pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine respiratory tract pathogens, and a reverse primer of a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-6 (sequence identifier number 1-6), and the PCR primer comprises forward and reverse PCR amplification primers of the rest thirteen respiratory tract pathogens, a forward and reverse amplification primer of a human DNA internal reference, a forward and reverse PCR amplification primer of a reaction internal reference, PCR amplification primers of the nine respiratory tract pathogens, and a PCR amplification primer of the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 7-44. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention discloses a primer composition for guiding nitroglycerin medication and healthy drinking, a multiple gene detection kit and a use method of the kit, and the kit comprises the primer composition, a PCR buffer solution and a positive reference substance, and the PCR buffer solution comprises ultrapure water, an X solution, a 10*PCR (polymerase chain reaction) buffer solution, a PCR primer, a 25mM magnesium chloride solution and DNA polymerase, the primer composition comprise two forward and reverse amplification primers of different gene types on the 2 SNP sites of genes related to the nitroglycerin medication and healthy drinking and forward and reverse amplification primers capable of reflecting internal reference, the gene sequences of the primers are represented as SEQ ID NO.1-NO.8; the use method comprises the step of acquiring a sample and extracting nucleic acid, the step of performing the PCR reaction by using extracted nucleic acid as a template, and the final step of separating the sample through capillary electrophoresis. The primer composition has the advantages of being strong in specificity, high in accuracy, high in flux, strong in reliability, low in cost and free from false negative result.

The invention discloses a kit for synchronously detecting fifteen hemorrhagic fever pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine hemorrhagic fever pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-10 (sequence identifier number 1-10), and the PCR primer comprises forward and reverse PCR amplification primers of the rest six hemorrhagic fever pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the nine hemorrhagic fever pathogens and the human RNA internal reference, and has a gene sequence show as SEQ ID NO. 10-36. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention discloses a method for early detection on a colorectal cancer on the basis of a NDRG4 gene methylation sequence. The auxiliary diagnosis is performed on the early colorectal cancer through detecting whether a section of methylation sequence of a NDRG4 gene promoter region is methylated or not; the method comprises the following steps: extracting DNA from tissue and fecal samples, treating with hydrosulfite, then performing real-time quantitative PCR determination and judging a methylation level through calculating whether an amplified Ct ratio difference value between a target gene NDRG4 and an internal reference gene B2M is within a decision critical value or not. The method is noninvasive and can be used for the auxiliary diagnosis of the early colorectal cancer.

The invention discloses a kit capable of synchronously detecting eighteen kinds of fever with eruption pathogens and a detection method thereof. The kit comprises diethylpyrocarbonate (DEPC) water, 5*reverse transcriptase (RT) buffer solution, reverse transcription primers, reverse transcriptase, X solution, 10* polymerase chain reaction (PCR) buffer solution, PCR primers, 25m M magnesium chloride solution, deoxyribonucleic acid (DNA) polymerase and positive reference substances. The kit is characterized in that the reverse transcription primers comprise five kinds of fever with eruption pathogens and ribonucleic acid (RNA) internal reference RT primers, and the sequences are shown as SEQ ID NO.1-NO.6. The PCR primers comprises the remaining thirteen kinds of fever with eruption pathogens, herpes simplex virus 1, 2 universal type, human DNA internal reference, forward and reverse PCR amplification primers of a reaction internal reference, and the five kinds of fever with eruption pathogens and PCR amplification primers of a human RNA internal reference. The gene sequence is shown as SEQ ID NO.7-NO.44. The kit has the advantages of being strong in specificity, high in sensitivity, high in flux, strong in reliability, low in cost, and free from false-negative results.

The invention discloses a primer combination for guiding the application of a beta-receptor blocker, a multi-gene detection kit and a using method thereof. The kit disclosed by the invention comprises ultrapure water, an X solution, 10*PCR (Polymerase Chain Reaction) buffer, PCR primers, a 25 mM magnesium chloride solution, DNA (Deoxyribonucleic Acid) polymerase and a positive reference substance. The kit is characterized in that the primers include three forward and reverse amplification primers which are different in genotype and located on three SNPS loci on a gene related to the application of the beta-receptor blocker and forward and reverse amplification primers for the internal reference of reaction, and the gene sequences of the primers are shown in SEQ ID NO.1-NO.11. The using method comprises the following steps: collecting samples and extracting a nucleic acid; carrying out a PCR reaction by taking the extracted nucleic acid as a template; finally, carrying out capillary ionophortic separation on the samples by using a GeXP genetic analyzer; the using method has the advantages of strong specificity, high accuracy, high flux, high reliability, low cost, no false negative results.

The invention discloses a primer composition and multiple-gene detection kit for guiding the administration of thiazine diuresis drugs and an application method thereof. The primer composition comprises ultrapure water, an X solution, a 10*PCR buffer solution, a PCR primer, a 25mM magnesium chloride solution, a DNA polymerase and a positive reference substance and is characterized in that the PCR primer comprises the following ten forward/reverse amplification primers and reaction reference forward/reverse amplification primers of different gene types on 15 SNP sites on the gene related to the administration of thiazine diuresis drugs, wherein the gene sequences are shown by the SEQ ID No.1 to No.46. The application method comprises the following steps: collecting the samples and extracting nucleic acid; performing a PCR reaction by taking the extracted nucleic acid as a template; and finally, performing capillary electrophoretic separation of the samples with the GeXP genetic analyzer. The primer composition has the advantages of strong specificity, high accuracy, high flux, high reliability, low cost and no false negative result.

The invention relates to the field of microbe experimental facilities, and in particular discloses a multi-function instrument used for the microbe acid and gas producing experiment and the sulfur-containing amino acid detection experiment. The multi-function instrument comprises a constant temperature incubator, a fermentation test tube, an acid and gas producing experiment indicating device and a sulfur-containing amino acid detection device, wherein the acid and gas producing experiment indicating device comprises a liquid storage chamber and a seal end cover, the middle part of the seal end cover sinks, the sinking part is in sealed connection with a gas collecting tube, a water injecting hole and an air hole are formed in the seal end cover, and a puncture device and a seal rubber sleeve are in sealed connection to the bottom of the liquid storage chamber; the sulfur-containing amino acid detection device comprises a detection chamber, a rubber plug is plugged to the lower opening of the detection chamber, a puncture device is inserted into the rubber plug, and a rubber seal sleeve is arranged at the lower end of the detection chamber; a filter paper strip coiled to form the barrel shape is plugged in the detection chamber. The multi-function instrument has the advantages that the size is small, the taking for use is convenient, the operation is simple, the efficiency is high, the space is saved, the experiment is simple and rapid, the result is reliable, and multiple purposes are realized.

The invention provides a target cell TCC-3T3 for inspecting killing effect of TCR-T (T cell receptor T) cell, and belongs to the technical field of cell engineering. The target cell TCC-3T3 is a mousefibroblast-like cell for expressing a target antigen gene of the TCR-T cell, an HLA (human leukocyte antigen) gene, and a fusion protein gene of Caveolin-1 and caspase-3. A preparation method of thetarget cell TCC-3T3 comprises the following steps of transferring the fusion protein gene of Caveolin-1 and caspase-3 into the mouse fibroblast-like cell by a lentiviral transfection method, so as toobtain the cell CC-3T3 for expressing the fusion protein; transferring the target antigen gene of the TCR-T cell and the HLA gene into the cell CC-3T3 by the lentiviral transfection method, so as to obtain the target cell TCC-3T3. The target cell TCC-3T3 has the advantages that the killing background is low; the target cell TCC-3T3 is sensitive to the killing signals; the specific killing abilityof effector cells can be accurately reflected.

The invention provides a target cell CCC-3T3 for inspecting killing effect of CAR-T (chimeric antigen receptor T) cell, and belongs to the technical field of cell engineering. The target cell CCC-3T3is a mouse fibroblast-like cell for expressing a target antigen gene of the CAR-T cell and a fusion protein gene of Caveolin-1 and caspase-3. A preparation method of the target cell CCC-3T3 comprisesthe following steps of transferring the fusion protein gene of Caveolin-1 and caspase-3 into the mouse fibroblast-like cell by a lentiviral transfection method, so as to obtain the cell CC-3T3 for expressing the fusion protein; transferring the target antigen gene of the CAR-T cell into the cell CC-3T3 by the lentiviral transfection method, so as to obtain the target cell CCC-3T3. The target cellCCC-3T3 has the advantages that the killing background is low; the target cell CCC-3T3 is sensitive to the killing signals; the specific killing ability of effect cells can be accurately reflected.

The invention discloses a high-throughput quantitative detection kit for food-borne pathogenic bacteria. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.36, of 9 kinds of food-borne pathogenic bacteria; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilledwater. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.

The invention discloses a high-throughput quantitative detection kit for pathogenic vibrio. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.32, of 8 kinds of pathogenic vibrio; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilled water. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.

The invention discloses a high-throughput quantitative detection kit for diarrhea pathogenic bacteria. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.36, of 9 kinds of diarrhea pathogenic bacteria; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilled water. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.