43results about How to "No false negative results" patented technology

The invention discloses a multi-gene detection kit for guiding administration of 5-fluorouracil and a detection method of the multi-gene detection kit. The multi-gene detection kit comprises ultrapure water, an X solution, a 10*PCR (Polymerase Chain Reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (Deoxyribonucleic Acid) polymerase and a positive control, and is characterized in that the PCR primer comprises six forward and reverse amplification primers with different genotypes on seven SNP (Single Nucleotide Polymorphism) sites of genes related to the administration of 5-fluorouracil, and forward and reverse amplification primers of a DNA internal reference and a reaction internal reference, and gene sequences of the forward and reverse amplification primers are shown as SEQ ID NO. 1-32 (Sequence Identifier Numbers 1-32). The detection method of the multi-gene detection kit comprises the steps of collecting a sample, extracting nucleic acid, conducting a PCR by taking the extracted nucleic acid as a template, and conducting capillary electrophoretic separation on the sample with a GeXP genetic analyser. The multi-gene detection kit and the detection method have the advantages of strong specificity, high accuracy, flux and reliability, low cost and no false negative result.

The invention discloses a kit for synchronously detecting twenty-two respiratory tract pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine respiratory tract pathogens, and a reverse primer of a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-6 (sequence identifier number 1-6), and the PCR primer comprises forward and reverse PCR amplification primers of the rest thirteen respiratory tract pathogens, a forward and reverse amplification primer of a human DNA internal reference, a forward and reverse PCR amplification primer of a reaction internal reference, PCR amplification primers of the nine respiratory tract pathogens, and a PCR amplification primer of the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 7-44. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention discloses a primer composition, a kit and a use method for detecting the expression level of insulin-like peptide ILP series genes in Drosophila melanogaster. The primer composition comprises RT amplification primers and PCR amplification primers of insulin-like peptide and RNA internal reference. The kit includes DEPC water, 5×RT buffer, reverse transcription primer, reverse transcriptase, Z solution, 10×PCR buffer, PCR primer, 25mM magnesium chloride solution, DNA polymerase and positive control. The present invention can detect 7 insulin-like peptides of Drosophila melanogaster at the same time, and can complete the detection of 192 samples in one day, which not only saves production and detection costs, but also improves detection efficiency; the RNA internal reference provides a control internal reference for the integrity of the sample RNA, Ensure the judgment of sample quality during the inspection process, avoid false negatives, and make the detection have better sensitivity and specificity, thereby avoiding the problem of low specificity of other detection methods.

The invention discloses a kit for synchronously detecting related gene expression level of 14 antitumor drugs by using a paraffin embedding biopsy sample, and a detection method thereof. The kit comprises DEPC (diethylpyrocarbonate) water, 5*RT buffer solution, a reverse transcription primer, a reverse transcription enzyme, an X solution, 10*PCR buffer solution, a PCR primer, 25mM magnesium chloride solution, DNA (deoxyribonucleic acid) polymerase and a positive reference substance; the reverse transcription primer comprises related genes of the 14 antitumor drugs, and RT amplification primers of an RNA (ribonucleic acid) internal reference; gene sequences are shown in SEQ ID NO.1 to NO.18; the PCR primer includes related genes of the 14 antitumor drugs, and positive and negative PCR amplification primers of a DNA internal reference; the gene sequences are shown in SEQ ID NO.19 to NO.38. The kit has the advantages of strong specificity, high sensitivity, high flux, strong reliability, low cost and absence of false-negative result.

The invention discloses a kit capable of synchronously detecting eighteen kinds of fever with eruption pathogens and a detection method thereof. The kit comprises diethylpyrocarbonate (DEPC) water, 5*reverse transcriptase (RT) buffer solution, reverse transcription primers, reverse transcriptase, X solution, 10* polymerase chain reaction (PCR) buffer solution, PCR primers, 25m M magnesium chloride solution, deoxyribonucleic acid (DNA) polymerase and positive reference substances. The kit is characterized in that the reverse transcription primers comprise five kinds of fever with eruption pathogens and ribonucleic acid (RNA) internal reference RT primers, and the sequences are shown as SEQ ID NO.1-NO.6. The PCR primers comprises the remaining thirteen kinds of fever with eruption pathogens, herpes simplex virus 1, 2 universal type, human DNA internal reference, forward and reverse PCR amplification primers of a reaction internal reference, and the five kinds of fever with eruption pathogens and PCR amplification primers of a human RNA internal reference. The gene sequence is shown as SEQ ID NO.7-NO.44. The kit has the advantages of being strong in specificity, high in sensitivity, high in flux, strong in reliability, low in cost, and free from false-negative results.

The invention discloses a kit for synchronously detecting thirteen diarrhea viruses and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the eleven diarrhea RNA viruses and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-12 (sequence identifier number 1-12), and the PCR primer comprises forward and reverse PCR amplification primers of the rest two diarrhea viruses, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the eleven diarrhea RNA viruses and the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 13-32. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention discloses an SNP (Single Nucleotide Polymorphism) detection kit for guiding administration of platinum drugs and a detection method of the detection kit. The detection kit comprises an X solution, a 10*PCR (Polymerase Chain Reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (Deoxyribonucleic Acid) polymerase and a positive control, and is characterized in that the PCR primer comprises six forward and reverse amplification primers with different genotypes on eight SNP sites of genes related to the administration of the platinum drugs, and forward and reverse amplification primers of a human DNA internal reference and a reaction internal reference, and gene sequences of the forward and reverse amplification primers are shown as SEQ ID NO. 1-36 (Sequence Identifier Numbers 1-36). The detection method of the detection kit comprises the steps of collecting a sample, extracting nucleic acid, conducting a PCR by taking the extracted nucleic acid as a template, and conducting capillary electrophoretic separation on the sample with a GeXP genetic analyser. The detection kit and the detection method have the advantages of strong specificity, high accuracy, flux and reliability, low cost and no false negative result.

The invention provides a target cell TCC-3T3 for testing the killing effect of TCR-T cells, belonging to the technical field of cell engineering; the target cell TCC-3T3 expresses TCR-T cell target antigen gene, HLA gene, Caveolin- 1 and caspase‑3 fusion protein gene in mouse fibroblast-like cells. The preparation method of the target cell TCC-3T3: using the lentivirus transfection method to transfer the fusion protein gene of Caveolin-1 and caspase-3 into mouse fibroblast-like cells to obtain CC-3T3 cells expressing the fusion protein; then The TCR-T cell target antigen gene and HLA gene were transferred into CC-3T3 cells by lentivirus transfection method to obtain the target cell TCC-3T3. The target cell TCC-3T3 has low killing background, is sensitive to killing signals, and can accurately reflect the specific killing ability of effector cells.