The present invention discloses a low-noise single-gate non-volatile memory and an operation method thereof, wherein a transistor and a capacitor structure are embedded in a semiconductor substrate; the electrically-conductive gate of the transistor and the electrically-conductive gate of the capacitor structure are interconnected to form a single floating gate of a memory cell; an ion-doped buried layer is formed between the dielectric layer of the capacitor structure and the semiconductor substrate to reduce the external interference on the capacitor structure and control the initial threshold voltage; a reverse bias may be used to implement the reading, writing, and erasing operations of the single-floating-gate memory cell; in the operation of the low-noise single-gate non-volatile memory having an isolation well, positive and negative voltages may be applied to the drain, the gate, and the silicon substrate/the isolation well to create an inversion layer, and thereby, the absolute voltage, the area of the voltage booster circuit, and the current consumption can be reduced.