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Lactobacillus gasseri for preventing and/or treating genital tract flora disorder related diseases

A technology of Lactobacillus gasseri, bacterial suspension, applied in the direction of Lactobacillus, microorganism-based methods, sexual diseases, etc., can solve the problem of increasing the risk of repeated infection and recurrence, unfavorable reproductive tract to maintain homeostasis, and easy damage to liver and kidney functions. and other problems, to achieve the effect of treating or preventing reproductive tract microbial infections, reducing the recurrence rate and improving the cure rate.

Pending Publication Date: 2021-03-09
SHENZHEN HUADA GENE INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in antibiotic drug treatment: first, antibiotics kill pathogenic bacteria and eliminate inflammation while inhibiting the growth of probiotics, reducing the abundance of probiotics, which is very unfavorable for the reproductive tract to maintain a stable state, and greatly increases the risk of repeated infection. The risk of infection and recurrence; second, taking drugs cannot achieve the purpose of precise treatment, and can only act on a broad-spectrum systemic range, which is especially easy to damage liver and kidney functions; third, frequent use of antibiotics can easily lead to drug resistance of pathogenic bacteria, This is also an important reason for the secondary infection

Method used

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  • Lactobacillus gasseri for preventing and/or treating genital tract flora disorder related diseases
  • Lactobacillus gasseri for preventing and/or treating genital tract flora disorder related diseases
  • Lactobacillus gasseri for preventing and/or treating genital tract flora disorder related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Isolation and identification of Lactobacillus gasseri (Lactobacillus gasseri) TM13-16

[0048] 1. Sample collection

[0049] The isolated sample comes from the stool of a healthy person. The stool is collected into a sterile sample tube and brought back to the laboratory for sorting within 1 hour.

[0050] 2. Separation and purification

[0051] Immediately transfer the collected fresh samples to an anaerobic operation box, take 0.2g of the sample in 1ml of sterile PBS (phosphate buffer), shake and mix well, and then carry out gradient dilution coating, the medium adopts the modified version of PYG medium , the specific formula is (1L): tryptone 8g, soybean peptone 2g, polyprotein 1g, casein 1g, yeast powder 10g, beef extract 5g, glucose 5g, K 2 HPO 4 2g, maltose 0.5g, cellobiose 0.5g, soluble starch 0.5g, sodium sulfide 0.25g, Tween 800.5mL, Cysteine-HCl·H 2 O 0.5g, glycerol 0.5mL, sodium acetate 5g, heme 5mg, vitamin K 11μL, inorganic salt solution (ev...

Embodiment 2

[0064] Example 2: Genome sequencing and species classification and functional gene analysis of Lactobacillus gasseri (Lactobacillus gasseri) TM13-16

[0065] 1. Genome sequencing

[0066] Centrifuge the overnight cultured TM13-16 bacterial solution at 7,227 g at 4°C for 10 minutes, resuspend the resulting pellet in 1 mL of Tris-EDTA, add 50 μL of 10% SDS and 10 μL of proteinase K (20 mg / mL) at 55°C The cells were lysed in a water bath for 2 hours, and the DNA was extracted by the phenol-chloroform method. And use the Illumina Hiseq 2000 platform to sequence it, the sequencing length is 500bp in both directions, and use SOAPdenovo to assemble the reads. After evaluation, use GCskew to analyze the GC content of the whole genome and visualize the whole genome sequence and functional distribution ( figure 1 ).

[0067] 2. Classification of strain genome species

[0068] Use Checkm software to compare and analyze the whole genome sequence, and get the species information closes...

Embodiment 3

[0071] Example 3: Bioactive substances produced by Lactobacillus gasseri (Lactobacillus gasseri) TM13-16

[0072] The bioactive substances of TM13-16 mainly examine the content of L-lactic acid, D-lactic acid and hydrogen peroxide in metabolites.

[0073] 1. Sample pretreatment

[0074] The strain TM13-16 was inoculated into MRS medium, and cultured at 37° C. for 24 h under aerobic and anaerobic conditions, respectively.

[0075] Take 1mL of the bacterial solution and centrifuge at 8000r / min for 5min, and take the supernatant to detect the content of L-lactic acid and D-lactic acid.

[0076] Then take 1 mL of the bacterial solution and add lysozyme (final concentration: 1 mg / mL), let stand at 37°C for 15 min, centrifuge at 8000 r / min for 5 min, and take the supernatant to test the concentration of hydrogen peroxide.

[0077] 2. Measurement method

[0078] The contents of L-lactic acid and D-lactic acid were measured using L-Lactic Acid (L-Lactate) Assay Kit and D-Lactic Aci...

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Abstract

The invention relates to the technical field of biology, in particular to a novel lactobacillus gasseri strain for preventing and / or treating genital tract infection and / or genital tract flora disorder and application of the novel lactobacillus gasseri strain. In order to solve the technical problems of different degrees of side effects, high recurrence rate or large trauma and the like of the existing medicine for treating genital tract infection, the invention screens out a new lactobacillus gasseri TM13-16 for preventing and / or treating genital tract infection and / or genital tract flora disorder, which can generate secretions such as lactic acid, hydrogen peroxide and the like is used for inhibiting the growth of pathogenic bacteria of genital tract infection, is used for preventing and / or treatment genital tract infection diseases, particularly reducing the recurrence rate, has small toxic and side effects and lasting efficacy, and can be used as dominant lactobacillus for vagina to repair flora disorder and restore vaginal homeostasis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new strain of Lactobacillus gasseri for preventing and / or treating reproductive tract infection and its application. Background technique [0002] The environment of the female reproductive tract is often affected by fluctuations in hormone levels, poor sanitation control, seasonal changes, and even emotional instability, resulting in disordered flora, which in turn leads to the invasion of pathogenic bacteria, causing microbial infections such as bacteria, molds, and viruses. Common There are Escherichia coli, Gardnerella, Candida albicans and HPV viruses. The clinical symptoms of reproductive tract infection are: vaginal itching and burning, abnormal discharge, frequent urination and painful urination, which can lead to premature delivery and cervical cancer in severe cases. According to statistics, the infection rate of reproductive tract microorganisms in women of childbearing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P15/02A61P31/04A61P31/10A61P33/02A61P31/20A61P31/22A23L33/135A23C9/123C12R1/225
CPCA61K35/747A61P15/02A61P31/04A61P31/10A61P33/02A61P31/20A61P31/22A23L33/135A23C9/1234A23V2002/00A23V2400/145A23V2200/30
Inventor 张笑薇吕金丽赵少伟邹远强肖亮
Owner SHENZHEN HUADA GENE INST
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