The invention discloses a measuring method of the coupling relation of the human body upper limb shoulder glenohumeral joint rotation center and the upper arm lifting angle. The measuring method comprises the steps of acquiring human body shoulder joint motion data, wherein mark point motion blocks are fixed to the scapula and the humerus, and six mark points are fixed to the 7 th cervical vertebra, the 8 th thoracic vertebra, the jugular notch, the xiphoid, the lateral epicondyle and the medial epicondyle of the human body to obtain motion information of corresponding bones; building a shoulder motion model, wherein the scapula and the humerus are used as a rigid body respectively, and based on rotation geometric knowledge, a kinematic model of which the rotation center is unchanged relative to the adjacent rigid body pose is built; solving the coupling relation of the glenohumeral joint rotation center and the lifting angle, wherein since the joint center is unchanged relative to themark point pose of the adjacent rigid body, regression parameters are obtained, then the instantaneous position of the rotation center is obtained, and thus the coupling relation of the lifting angleand the rotation center is obtained. The measuring points are bond or fixed to the human body through a flange, deformation is reduced, calculation is simple, and measurement is accurate.