The invention belongs to the technical field of medical instruments, and relates to a using method of a vector positioning puncture auxiliary device. The intercostal number is taken as a horizontal axis, seven body surface marking lines of a body surface sternum line, a clavicle midline, an anterior axillary line, a midaxillary line, a posterior axillary line, a scapular line and a posterior median line are taken as vertical axis, scan data are analyzed based on a chest CT scan image, the maximum cross-sectional image of a target point is taken to determine the intercostal number and the bodysurface marking lines which are similar to the maximum cross-sectional image, the intercostal number is the puncture intercostal number, the parallel distance between a puncture position of the maximum cross-sectional image of the closest body surface marking line is measured to determine a body surface puncture point, the center of the target point and the puncture point are connected on the maximum cross-sectional image of the target point to determine the puncture angle, and the distance between the body surface puncture point and the target point is measured as the inserting depth of the puncture needle. The puncture accuracy is improved, the work intensity of doctors is reduced, the radiation damage of the doctors and patients is reduced, the method is simple and easy to operate, safeand reliable, good in versatility, easy to operate and popularize and friendly in application environment.