The invention belongs to the technical field of shoulder health care and nursing and particularly relates to an exquisite shoulder beautifying and conditioning formula, a preparation method and a use method. The formula comprises a massage oil component and a tightening oil component, wherein the massage oil component is prepared from grapefruit oil, rosemary leaf oil and grape seed oil, and the tightening oil component is prepared from lavender oil, propylene glycol, pelargonium graveolens flower oil, glycerol, grapefruit oil, rhizoma cyperi extractive and anise oil. The exquisite shoulder beautifying and conditioning formula disclosed by the invention has overall effects of activating shoulder blood vessels, tightening skin, enabling the skin to be elastic, removing redundant fat of the shoulder, effectively relieving shoulder fatigue and shoulder muscle stiffness, improving human body immunity, effectively preventing shoulder diseases, improving human body vigor, improving sleeping quality, having no toxicity and harm to a human body, having no side effect and having effects of thinning shoulders, beautifying shoulders, improving skin and preventing shoulder diseases.