The invention discloses a wormwood non-woven fabric which is prepared from, by weight, 65-80% of chemical fiber with low melting point, 15-20% of wormwood fiber and 5-15% of wormwood particles, wherein the length of wormwood fiber ranges from 1 to 90 mm and the fineness thereof ranges from 3D to 60D, the length of chemical fiber with low melting point ranges from 10 mm to 200 mm and the fineness thereof ranges from 3D to 100D, and the wormwood particles are of grain size smaller than 80 meshes. The invention further discloses a manufacturing method of the wormwood non-woven fabric, including the steps of unpacking, coarse opening, fine opening, screening and drying, metering of chemical fiber, metering of wormwood fiber, primary mixing, uniform mixing, pre-laid process, air-laid process, heating to melt, cold pressing, and cooling. The wormwood non-woven fabric manufactured by the method has good breathability and also has functions of mosquito preventing and mite repelling.