The invention relates to the technical field of wood floor detection, in particular to a wood floor production and manufacturing flatness detection device which comprises a detection table, a driving mechanism and a detection mechanism, the driving mechanism is arranged on the detection table, and the detection mechanism is arranged between the left side and the right side of the driving mechanism. The detection mechanism comprises a U-shaped frame, a fixed pipe, a mounting frame, a roller, a first elastic telescopic rod, a mounting block, a marking pipe and a marking branch chain. When flatness detection is carried out on the wood floor, the deformation area of the wood floor can be distinguished and marked, so that the deformation area can be quickly found out when the wood floor is repaired in the later period, the flatness of the surface of the wood floor can be carefully detected, and missing detection of partial area of the surface of the wood floor is prevented, the device can detect the flatness of a plurality of wood floors at the same time, can visually find whether the thicknesses of the wood floors are the same or not in the detection process, and quickly finds out the wood floors with large thickness differences.